Publications — Newcotiana

Trends in GeneticsTan, J., Forner, J., Karcher, D., Bock, R. (2022) DNA base editing in nuclear and organellar genomes.
Frontiers in Plant ScienceGöritzer, K,, Grandits, M,, Grünwald-Gruber, C,, Figl, R,, Mercx, S,, Navarre, C,, Ma, JK-C., Teh AY-H. (2022) Engineering the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9.
ACS Synthetic BiologyMoreno-Giménez, E., Selma, S., Calvache, C., Orzáez, D. (2022) GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants
Nature CommunicationsPei, L., Garfinkel, M. & Schmidt, M. (2022) Bottlenecks and opportunities for synthetic biology biosafety standards.
BMC BiotechnologyGarcia-Perez, E., Diego-Martin, B., Quijano-Rubio, A., Moreno-Giménez, E., Selma, S., Orzáez, D., Vázquez-Vilar, M. (2022) A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana.
The Plant JournalDrapal, M., Enfissi, E.M.A. and Fraser, P.D. (2022) The chemotype core collection of genus Nicotiana.
Biotechnology and BioengineeringPinneh, E. C., Dolleweerd, C. J., Göritzer, K., Drake, P. M. W., Ma, J. K-C., & Teh, A. Y-H. (2022) Multiple gene expression in plants using MIDAS-P, a versatile type II restriction-based modular expression vector.
CellsPizzio, G.A.; Mayordomo, C.; Lozano-Juste, J.; Garcia-Carpintero, V.; Vazquez-Vilar, M.; Nebauer, S.G.; Kaminski, K.P.; Ivanov, N.V.; Estevez, J.C.; Rivera-Moreno, M.; Albert, A.; Orzaez, D.; Rodriguez, P.L. (2022) PYL1- and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue.
Plant Biotechnology JournalCalvache, C., Vazquez-Vilar, M., Selma, S., Uranga, M., Fernández-del-Carmen, A., Daròs, J.-A. and Orzáez, D. (2022) Strong and tunable anti-CRISPR/Cas activities in plants.
Plant PhysiologyFrusciante, S., Demurtas, O.C., Sulli, M., Mini, P., Aprea, G., Diretto, G., Karcher, D., Bock, R., Giuliano, G. (2021) Heterologous expression of Bixa orellana cleavage dioxygenase 4–3 drives crocin but not bixin biosynthesis.
Journal of Virological MethodsPeyret, H., Groppelli, E., Clark, D., Eckersley, N., Planche, T., Ma, J., Lomonossoff, GP. (2021) Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics.
The CRISPR JournalUranga, M., Vazquez-Vilar, M., Orzáez, D., Daròs, J,A. (2021) CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors.
Frontiers in Plant ScienceVazquez-Vilar, M., Garcia-Carpintero, V., Selma, S., Bernabé-Orts, J.M., Sanchez-Vicente, J., Salazar-Sarasua, B., Ressa, A., de Paola, C., Ajenjo, M., Quintela, J.C., Fernández-del-Carmen, A., Granell, A. and Orzáez, D. (2021) The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants.
Plant Biotechnology JournalLobato Gómez, M., Huang, X., Alva- rez, D., He, W., Baysal, C., Zhu, C., Armario Najera, V., Blanco Perera, A., Cerda Bennasser, P., Andera Saba-Mayoral, A., [...], Christou, P. (2021) Contributions of the international plant science community to the fight against human infectious diseases – part 1: epidemic and pandemic diseases.
Plant Biotechnology JournalHe, W., Baysal, C., Lobato-Gómez, M., Huang, X., Alvarez, D., Zhu, C., Armario Najera, V., Blanco Perera, A., Cerda Bennasser, P., Saba-Mayoral, A., [...], Capell, T. (2021) Contributions of the international plant science community to the fight against infectious diseases in humans-part 2: Affordable drugs in edible plants for endemic and re-emerging diseases.
Transgenic ResearchDrapal, M., Enfissi, E.M.A., Fraser, P.D. (2021) Metabolic effects of agro-infiltration on N. benthamiana accessions.
Transgenic ResearchHuang, TK., Puchta, H. (2021) Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering.
Transgenic ResearchBuyel, J.F., Stöger, E. & Bortesi, L. (2021) Targeted genome editing of plants and plant cells for biomanufacturing.
Journal of Plant PhysiologySchillberg, S., Finnern, R. (2021) Plant molecular farming for the production of valuable proteins – Critical evaluation of achievements and future challenges.
Plant Biotechnology JournalHuang, TK., Armstrong, B., Schindele, P., Puchta, H. (2021) Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a.
The Plant JournalUranga, M., Aragonés, V., Selm,a S., Vázquez-Vilar, M., Orzáez, D., Daròs, J.A. (2021) Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector
Frontiers in Plant ScienceMenary, J., Amato, M., Sanchez, A.C., Hobbs, M., Pacho, A., Fuller, S.S. (2020) New Hope for a "Cursed" Crop? Understanding Stakeholder Attitudes to Plant Molecular Farming With Modified Tobacco in Europe.
Frontiers in Plant ScienceDiego-Martin, B., González, B., Vazquez-Vilar, M., Selma, S., Mateos-Fernández, R., Gianoglio, S., Fernández-Del-Carmen, A., Orzáez, D. (2020) Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities.
Plant Biotechnology JournalMerker, L., Schindele, P., Huang, T.K., Wolter, F., Puchta, H. (2020) Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature‐tolerant CRISPR/LbCas12a.
PLoS ONEMenary, J., Hobbs, M., Mesquita de Albuquerque, S., Pacho, A., Drake, P.M.W., Prendiville, A., Ma, J.K-C., Fuller, S.S. (2020) Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products.
Nature CommunicationsTan, J., Zhang, F., Karcher, D., Bock, R. (2020) Expanding the genome-targeting scope and the site selectivity of high-precision base editors.
Plant JournalFraser, P.D., Aharoni, A., Hall, R.D., Huang, S., Giovannoni, .JJ., Sonnewald, U., Fernie, A.R. (2020) Metabolomics should be deployed in the identification and characterization of gene-edited crops.
Plant CellDemurtas, OC., de Brito Francisco, R., Diretto, G., Ferrante, P., Frusciante, S., Pietrella, M., Aprea, G., Borghi, L., Feeney, M., Frigerio, L., Coricello, A., Costa, G., Alcaro, S., Martinoia, E., Giuliano, G. (2019) ABCC Transporters Mediate the Vacuolar Accumulation of Crocins in Saffron Stigmas.
Plant Biotechnology Journal Schindele, P., Puchta, H. (2019) Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing.
Plant Biotechnology Journal Bernabé‐Orts, J.M., Casas‐Rodrigo, I., Minguet , E.G., Landolfi , V., Garcia‐Carpintero, V., Gianoglio, S., Vázquez‐Vilar, M., Granell , A., Orzaez, D. (2019) Assessment of Cas12a‐mediated gene editing efficiency in plants.
Plant Biotechnology Jutras, P. V., Grosse‐Holz, F., Kaschani , F., Kaiser, M., Michaud, D., van der Hoorn, R.A.L. (2019) Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana.
Front. Plant SciSchillberg S., Raven N., Spiegel H., Rasche S., Buntru M. (2019) Critical analysis of the commercial potential of plants for the production of recombinant proteins.
Metabolic EngineeringNogueira, M., Enfissi, E.M.A. Welsch, R., Beyer, P. Zurbriggen, MD., and Fraser, PD. (2019). The construction of a fusion enzyme for astaxanthin formation and its characterisation in microbial and plant hosts: A new tool for engineering ketocarotenoids.
Nature CommunicationsTan, J., Zhang, F., Karcher, D., Bock, R. (2019) Engineering of high-precision base editors for site-specific single nucleotide replacement.
Current Opinion in BiotechnologyPei L., Schmidt, M. (2019) Novel biotechnological approaches to produce biological compounds: challenges and opportunities for science communication.
Plant Molecular BiologyHoelscher, M., Tiller, N., Teh, A.YH., Wu, G-Z., Ma, J. K-C., Bock, R. (2018) High-level expression of the HIV entry inhibitor griffithsin from the plastid genome and retention of biological activity in dried tobacco leaves.